dc.contributor.author | Zhang, Zhibo | |
dc.contributor.author | Wang, Qiao-Chun | |
dc.contributor.author | Spetz, Carl | |
dc.contributor.author | Blystad, Dag-Ragnar | |
dc.date.accessioned | 2020-01-13T08:55:36Z | |
dc.date.available | 2020-01-13T08:55:36Z | |
dc.date.created | 2019-02-26T18:55:01Z | |
dc.date.issued | 2019-01-25 | |
dc.identifier.citation | Scientia Horticulturae. 2019, 249 7-14. | nb_NO |
dc.identifier.issn | 0304-4238 | |
dc.identifier.uri | http://hdl.handle.net/11250/2635853 | |
dc.description.abstract | Potato (Solanum tuberosum L.) is one of the most important crops grown in Norway, and virus-free plants are required for commercial potato production and for preservation of potato germplasm. The present study evaluates three in vitro therapies – meristem culture, cryotherapy, and chemotherapy combined with thermotherapy – to eliminate viruses from eight historically valuable potato cultivars belonging to the Norwegian potato germplasm. Potato virus Y, potato virus M, potato virus X and potato virus S were present in eight selected old potato cultivars due to long-term conservation in open field. Double-antibody sandwich enzyme-linked immunological assay (DAS-ELISA) and biological indicators were the standard tests used to confirm virus infection in our study. Six virus-free plants from four potato cultivars were obtained after meristem culture, and no virus-free potato cultivars were obtained after cryotherapy. Virus-free frequency for eight different potato cultivars after combining chemotherapy with thermotherapy varied from 36.4% to 100%, with single virus elimination rates of between 74.2% and 92.9%. Chemotherapy compared with thermotherapy was the most effective of the three in vitro therapies used in this study. Highly sensitive small RNA high-throughput sequencing (HTS) was used to evaluate the virus status of potato virus-free materials after virus eradication, and no virus was found, which was consistent with the results of DAS-ELISA and biological indicators. Small RNA HTS has been reported for the first time to evaluate the virus status after virus elimination and to control virus-free potato nuclear stocks. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.subject | Chemotherapy | nb_NO |
dc.subject | Cryotherapy | nb_NO |
dc.subject | Next generation sequencing | nb_NO |
dc.subject | Potato | nb_NO |
dc.subject | Thermotherapy | nb_NO |
dc.subject | Virus eradication | nb_NO |
dc.title | In vitro therapies for virus elimination of potato-valuable germplasm in Norway | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.rights.holder | © 2019 Elsevier B.V. All rights reserved | nb_NO |
dc.subject.nsi | VDP::Landbruks- og Fiskerifag: 900 | nb_NO |
dc.source.pagenumber | 7-14 | nb_NO |
dc.source.volume | 249 | nb_NO |
dc.source.journal | Scientia Horticulturae | nb_NO |
dc.identifier.doi | 10.1016/j.scienta.2019.01.027 | |
dc.identifier.cristin | 1680913 | |
dc.relation.project | Norges forskningsråd: 233787 | nb_NO |
dc.relation.project | Andre: FA1407 - DIVAS | nb_NO |
cristin.unitcode | 7677,3,0,0 | |
cristin.unitname | Divisjon for bioteknologi og plantehelse | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |