dc.contributor.author | Mageroy, Melissa | |
dc.contributor.author | Lachance, Denis | |
dc.contributor.author | Jancsik, Sharon | |
dc.contributor.author | Parent, Genevieve | |
dc.contributor.author | Seguin, Armand | |
dc.contributor.author | Mackay, John | |
dc.contributor.author | Bohlmann, Joerg | |
dc.date.accessioned | 2018-03-13T14:33:03Z | |
dc.date.available | 2018-03-13T14:33:03Z | |
dc.date.created | 2018-01-11T14:05:20Z | |
dc.date.issued | 2017-07-07 | |
dc.identifier.citation | PeerJ. 2017, 2017 (5), . | nb_NO |
dc.identifier.issn | 2167-8359 | |
dc.identifier.uri | http://hdl.handle.net/11250/2490347 | |
dc.description.abstract | Eastern spruce budworm (Choristoneura fumiferiana Clemens) (ESBW) is a major forest pest which feeds on young shoots of white spruce (Picea glauca) and can cause landscape level economic and ecological losses. Release of acetophenone metabolites, piceol and pungenol, from their corresponding glycosides, picein and pungenin, can confer natural resistance of spruce to ESBW. A beta-glucosidase gene, Pgβglu-1, was recently discovered and the encoded enzyme was characterized in vitro to function in the release of the defensive acetophenone aglycons. Here we describe overexpression of Pgβglu-1 in a white spruce genotype whose metabolome contains the glucosylated acetophenones, but no detectable amounts of the aglycons. Transgenic overexpression of Pgβglu-1 resulted in release of the acetophenone aglycons in planta. This work provides in vivo evidence for the function of Pgβglu-1. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | PeerJ | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | Picea glauca | nb_NO |
dc.subject | Conifer defense | nb_NO |
dc.subject | Spruce budworm | nb_NO |
dc.subject | Beta-glucosidase | nb_NO |
dc.subject | Transgenic spruce | nb_NO |
dc.title | In vivo function of Pgβglu-1 in the release of acetophenones in white spruce | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.rights.holder | © 2017 Mageroy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. | nb_NO |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 | nb_NO |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492 | nb_NO |
dc.source.pagenumber | 11 | nb_NO |
dc.source.volume | 2017 | nb_NO |
dc.source.journal | PeerJ | nb_NO |
dc.source.issue | 5 | nb_NO |
dc.identifier.doi | 10.7717/peerj.3535 | |
dc.identifier.cristin | 1540870 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |