Vis enkel innførsel

dc.contributor.authorZhang, Zhibo
dc.contributor.authorWang, Qiao-Chun
dc.contributor.authorSpetz, Carl
dc.contributor.authorBlystad, Dag-Ragnar
dc.date.accessioned2020-01-13T08:55:36Z
dc.date.available2020-01-13T08:55:36Z
dc.date.created2019-02-26T18:55:01Z
dc.date.issued2019-01-25
dc.identifier.citationScientia Horticulturae. 2019, 249 7-14.nb_NO
dc.identifier.issn0304-4238
dc.identifier.urihttp://hdl.handle.net/11250/2635853
dc.description.abstractPotato (Solanum tuberosum L.) is one of the most important crops grown in Norway, and virus-free plants are required for commercial potato production and for preservation of potato germplasm. The present study evaluates three in vitro therapies – meristem culture, cryotherapy, and chemotherapy combined with thermotherapy – to eliminate viruses from eight historically valuable potato cultivars belonging to the Norwegian potato germplasm. Potato virus Y, potato virus M, potato virus X and potato virus S were present in eight selected old potato cultivars due to long-term conservation in open field. Double-antibody sandwich enzyme-linked immunological assay (DAS-ELISA) and biological indicators were the standard tests used to confirm virus infection in our study. Six virus-free plants from four potato cultivars were obtained after meristem culture, and no virus-free potato cultivars were obtained after cryotherapy. Virus-free frequency for eight different potato cultivars after combining chemotherapy with thermotherapy varied from 36.4% to 100%, with single virus elimination rates of between 74.2% and 92.9%. Chemotherapy compared with thermotherapy was the most effective of the three in vitro therapies used in this study. Highly sensitive small RNA high-throughput sequencing (HTS) was used to evaluate the virus status of potato virus-free materials after virus eradication, and no virus was found, which was consistent with the results of DAS-ELISA and biological indicators. Small RNA HTS has been reported for the first time to evaluate the virus status after virus elimination and to control virus-free potato nuclear stocks.nb_NO
dc.language.isoengnb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectChemotherapynb_NO
dc.subjectCryotherapynb_NO
dc.subjectNext generation sequencingnb_NO
dc.subjectPotatonb_NO
dc.subjectThermotherapynb_NO
dc.subjectVirus eradicationnb_NO
dc.titleIn vitro therapies for virus elimination of potato-valuable germplasm in Norwaynb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© 2019 Elsevier B.V. All rights reservednb_NO
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900nb_NO
dc.source.pagenumber7-14nb_NO
dc.source.volume249nb_NO
dc.source.journalScientia Horticulturaenb_NO
dc.identifier.doi10.1016/j.scienta.2019.01.027
dc.identifier.cristin1680913
dc.relation.projectNorges forskningsråd: 233787nb_NO
dc.relation.projectAndre: FA1407 - DIVASnb_NO
cristin.unitcode7677,3,0,0
cristin.unitnameDivisjon for bioteknologi og plantehelse
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal