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dc.contributor.authorBlomstrand, Berit Marie
dc.contributor.authorEnemark, Heidi L
dc.contributor.authorØines, Øivind
dc.contributor.authorSteinshamn, Håvard
dc.contributor.authorAasen, Inga Marie
dc.contributor.authorMahnert, Karl-Christian
dc.contributor.authorSørheim, Kristin
dc.contributor.authorAthanasiadou, Spiridoula
dc.contributor.authorThamsborg, Stig Milan
dc.contributor.authorWoolsey, Ian
dc.identifier.citationParasitology Research. 2021, .en_US
dc.description.abstractThe widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesized that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture. Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents, were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at 7 different concentrations. Parasite growth inhibition was quantified by qPCR. The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regards to the dry matter concentration of each extract and to CT concentrations. Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum.en_US
dc.publisherSpringer Natureen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleExtracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell cultureen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© The Author(s) 2021en_US
dc.source.journalParasitology Researchen_US
dc.relation.projectNorges forskningsråd: 268264en_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal