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dc.contributor.authorNguyen, Nga
dc.contributor.authorSuokas, Marko
dc.contributor.authorKarppinen, Katja
dc.contributor.authorVuosku, Jaana
dc.contributor.authorJaakola, Laura
dc.contributor.authorHäggman, Hely
dc.identifier.citationScientific Reports. 2018, 8 .nb_NO
dc.description.abstractBilberry (Vaccinium myrtillus L.) fruits are an excellent natural resource for human diet because of their special favor, taste and nutritional value as well as medical properties. Bilberries are recognized for their high anthocyanin content and many of the genes involved in the anthocyanin biosynthesis have been characterized. So far, neither genomic nor RNA-seq data have been available for the species. In the present study, we de novo sequenced two bilberry fruit developmental stages, unripe green (G) and ripening (R). A total of 57,919 unigenes were assembled of which 80.2% were annotated against six public protein databases. The transcriptome served as exploratory data to identify putative transcription factors related to fruit ripening. Diferentially expressed genes (DEGs) between G and R stages were prominently upregulated in R stage with the functional annotation indicating their main roles in active metabolism and catalysis. The unigenes encoding putative ripening-related regulatory genes, including members of NAC, WRKY, LOB, ERF, ARF and ABI families, were analysed by qRTPCR at fve bilberry developmental stages. Our de novo transcriptome database contributes to the understanding of the regulatory network associated with the fruit ripening in bilberry and provides the frst dataset for wild Vaccinium species acquired by NGS technology.nb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleRecognition of candidate transcription factors related to bilberry fruit ripening by de novo transcriptome and qRT-PCR analysesnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.rights.holder© The Author(s) 2018nb_NO
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900nb_NO
dc.source.journalScientific Reportsnb_NO

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal