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dc.contributor.authorKarppinen, Katja
dc.contributor.authorAvetisyan, Anna
dc.contributor.authorHykkerud, Anne Linn
dc.contributor.authorJaakola, Laura
dc.date.accessioned2022-11-08T09:27:41Z
dc.date.available2022-11-08T09:27:41Z
dc.date.created2022-09-02T08:15:52Z
dc.date.issued2022-05-19
dc.identifier.citationFoods. 2022, 11 (10), .en_US
dc.identifier.issn2304-8158
dc.identifier.urihttps://hdl.handle.net/11250/3030577
dc.description.abstractBerries of the genus Vaccinium are highly valued health-beneficial superfoods, which are commonly subjected to adulteration and mixed with each other, or with other common berry species. A quantitative DNA-based method utilizing a chip-based digital polymerase chain reaction (dPCR) technique was developed for identifying and quantifying wild lingonberry (V. vitis-idaea) and cultivated American cranberry (V. macrocarpon). The dPCR method with species-specific primers for mini-barcoding was designed based on the indel regions found in the trnI-CAU–trnL-CAA locus in the chloroplast genome. The designed primers were able to amplify only target species, enabling to distinguish the two closely related species with good sensitivity. Our results illustrated the ability of the method to identify lingonberry and American cranberry DNA using PCR without the need for probes or further sequencing. The dPCR method could also quantify the DNA copy number in mixed samples. Based on this study, the method provides a basis for a simple, fast, and sensitive quantitative authentication analysis of lingonberry and American cranberry by dPCR. Moreover, it can also provide a platform for authentication analyses of other plant species as well by utilizing the indel regions of chloroplast genomes.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleA dPCR Method for Quantitative Authentication of Wild Lingonberry (Vaccinium vitis-idaea) versus Cultivated American Cranberry (V. macrocarpon)en_US
dc.title.alternativeA dPCR Method for Quantitative Authentication of Wild Lingonberry (Vaccinium vitis-idaea) versus Cultivated American Cranberry (V. macrocarpon)en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2022 by the authorsen_US
dc.source.pagenumber8en_US
dc.source.volume11en_US
dc.source.journalFoodsen_US
dc.source.issue10en_US
dc.identifier.doi10.3390/foods11101476
dc.identifier.cristin2048115
dc.source.articlenumber1476en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal